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Antigen Retrieval Solution (Alkaline)
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In immunohistochemistry, formalin/paraformaldehyde fixation cross-links proteins via aldehyde groups, masking antigen epitopes and impairing antibody binding. This alkaline antigen retrieval buffer restores epitopes through dual mechanisms: PH Neutralization: Dissociates formaldehyde-protein cross-links to unmask antigenic sites. Surfactant Activity: Reduces liquid surface tension, enhancing buffer penetration and optimizing antigen-antibody interaction efficiency.
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